Punch-excised explants of bovine mammary gland to model early immune response to infection.

ABSTRACT:

Background

Mammary gland (MG) infections (mastitis) are frequent diseases of dairy cows that affect milk quality, animal welfare and farming profitability. These infections are commonly associated with the bacteria Escherichia coli and Staphylococcus aureus. Different in vitro models have been used to investigate the early response of the MG to bacteria, but the role of the teat in mastitis pathogenesis has received less attention. In this study, we used punch-excised teat tissue as an ex vivo model to study the immune mechanisms that arise early during infection when bacteria have entered the MG.

Results

Cytotoxicity and microscopic analyses showed that bovine teat sinus explants have their morphology and viability preserved after 24 h of culture and respond to ex vivo stim

SUBMITTER: Noleto PG 

PROVIDER: S-EPMC10326946 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature