Inserting a Neuropixels probe into awake monkey cortex: two probes, two methods.

ABSTRACT: Neuropixels probes have revolutionized neurophysiological studies in the rodent, but inserting these probes through the much thicker primate dura remains a challenge. Here we describe two methods we have developed for the insertion of two types of Neuropixels probes acutely into the awake monkey cortex. For the fine rodent probe, which is unable to pierce native primate dura, we developed a dural-eyelet method to insert the probe repeatedly without breakage. For the thicker NHP probe, we developed an artificial dura system to insert the probe. We have now conducted successful experiments in 3 animals across 7 recording chambers with the procedures described here and have achieved stable recordings over several months in each case. Here we describe our hardware, surgical preparation, method

SUBMITTER: Namima T 

PROVIDER: S-EPMC10326968 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature