Glucose-stimulated KIF5B-driven microtubule sliding organizes microtubule networks in pancreatic β cells.

ABSTRACT: In pancreatic islet β cells, molecular motors use cytoskeletal polymers microtubules as tracks for intracellular transport of insulin secretory granules. The β-cell microtubule network has a complex architecture and is non-directional, which provides insulin granules at the cell periphery for rapid secretion response, yet to avoid over-secretion and subsequent hypoglycemia. We have previously characterized a peripheral sub-membrane microtubule array, which is critical for the withdrawal of excessive insulin granules from the secretion sites. Microtubules in β cells originate at the Golgi in the cell interior, and how the peripheral array is formed is unknown. Using real-time imaging and photo-kinetics approaches in clonal mouse pancreatic β cells MIN6, we now demonstrate that kinesin KIF5B

SUBMITTER: Bracey KM 

PROVIDER: S-EPMC10327020 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature