Engineering in vitro models of cystic fibrosis lung disease using neutrophil extracellular trap inspired biomaterials.

ABSTRACT: Cystic fibrosis (CF) is a muco-obstructive lung disease where inflammatory responses due to chronic infection result in the accumulation of neutrophil extracellular traps (NETs) in the airways. NETs are web-like complexes comprised mainly of decondensed chromatin that function to capture and kill bacteria. Prior studies have established excess release of NETs in CF airways increases viscoelasticity of mucus secretions and reduces mucociliary clearance. Despite the pivotal role of NETs in CF disease pathogenesis, current in vitro models of this disease do not account for their contribution. Motivated by this, we developed a new approach to study the pathobiological effects of NETs in CF by combining synthetic NET-like biomaterials, composed of DNA and histones, with an in vitro

SUBMITTER: Boboltz AM 

PROVIDER: S-EPMC10327088 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature