CaBLAM! A high-contrast bioluminescent Ca²⁺ indicator derived from an engineered Oplophorus gracilirostris luciferase.

ABSTRACT: Ca²⁺ plays many critical roles in cell physiology and biochemistry, leading researchers to develop a number of fluorescent small molecule dyes and genetically encodable probes that optically report changes in Ca²⁺ concentrations in living cells. Though such fluorescence-based genetically encoded Ca²⁺ indicators (GECIs) have become a mainstay of modern Ca²⁺ sensing and imaging, bioluminescence-based GECIs-probes that generate light through oxidation of a small-molecule by a luciferase or photoprotein-have several distinct advantages over their fluorescent counterparts. Bioluminescent tags do not photobleach, do not suffer from nonspecific autofluorescent background, and do not lead to phototoxicity since they do not require the extremely bright ex

SUBMITTER: Lambert GG 

PROVIDER: S-EPMC10327125 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature