The importance of input sequence set to consensus-derived proteins and their relationship to reconstructed ancestral proteins.

ABSTRACT: A protein sequence encodes its energy landscape - all the accessible conformations, energetics, and dynamics. The evolutionary relationship between sequence and landscape can be probed phylogenetically by compiling a multiple sequence alignment of homologous sequences and generating common ancestors via Ancestral Sequence Reconstruction or a consensus protein containing the most common amino acid at each position. Both ancestral and consensus proteins are often more stable than their extant homologs - questioning the differences and suggesting that both approaches serve as general methods to engineer thermostability. We used the Ribonuclease H family to compare these approaches and evaluate how the evolutionary relationship of the input sequences affects the properties of the resulting con

SUBMITTER: Nixon C 

PROVIDER: S-EPMC10327145 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature