Distinct functional constraints driving conservation of the cofilin N-terminal regulatory tail.

ABSTRACT: Cofilin family proteins have essential roles in remodeling the cytoskeleton through filamentous actin depolymerization and severing. The short unstructured N-terminal region of cofilin is critical for actin binding and harbors the major site of inhibitory phosphorylation. Atypically for a disordered sequence, the N-terminal region is highly conserved, but the aspects of cofilin functionality driving this conservation are not clear. Here, we screened a library of 16,000 human cofilin N-terminal sequence variants for their capacity to support growth in S. cerevisiae in the presence or absence of the upstream regulator LIM kinase. Results from the screen and subsequent biochemical analysis of individual variants revealed distinct sequence requirements for actin binding and regulation b

SUBMITTER: Sexton JA 

PROVIDER: S-EPMC10327202 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature