Abcg2a is the functional homolog of human ABCG2 expressed at the zebrafish blood-brain barrier.

ABSTRACT:

Background

A principal protective component of the mammalian blood-brain barrier (BBB) is the high expression of the multidrug efflux transporters P-glycoprotein (P-gp, encoded by ABCB1) and ABCG2 (encoded by ABCG2) on the lumenal surface of endothelial cells. The zebrafish P-gp homolog Abcb4 is expressed at the BBB and phenocopies human P-gp. Comparatively little is known about the four zebrafish homologs of the human ABCG2 gene: abcg2a, abcg2b, abcg2c, and abcg2d. Here we report the functional characterization and brain tissue distribution of zebrafish ABCG2 homologs.

Methods

To determine substrates of the transporters, we stably expressed each in HEK-293 cells and performed cytotoxicity and fluorescent efflux assays with kn

SUBMITTER: Thomas JR 

PROVIDER: S-EPMC10327217 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature