An efficient method for protoplast-mediated production of transformed castor bean (Ricinus communis) lines.

ABSTRACT:

Objective

The purpose of this study was to develop a method for the isolation, culture, and PEG-mediated protoplast transfection from leaves of in vitro-grown plants of Ricinus communis.

Results

Factors such as the enzymatic composition and the incubation time were evaluated. The enzymatic solution, containing 1.6% Cellulase-R10 and 0.8% Macerozyme-R10, with 16 h of incubation, was the best condition to achieve a high protoplast yield (481.16 × 10⁴ protoplasts/g FW) with a high percentage of viability (95%). The combination and concentration of enzymes have been shown to affect the protoplast isolation efficiency significantly. Furthermore, we found that a higher number of protoplasts (8.5 × 10⁵ protoplast/g FW) was obtained at a longer incubation time,

SUBMITTER: Figueroa-Varela P 

PROVIDER: S-EPMC10327310 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature