Ratiometric and discriminative visualization of autophagic processes with a novel dual-responded lysosome-specific fluorescent probe.

ABSTRACT:

Background

Autophagy is a critical self-eating pathway involved in numerous physiological and pathological processes. Lysosomal degradation of dysfunctional organelles and invading microorganisms is central to the autophagy mechanism and essential for combating disease-related conditions. Therefore, monitoring fluctuations in the lysosomal microenvironment is vital for tracking the dynamic process of autophagy. Although much effort has been put into designing probes for measuring lysosomal viscosity or pH separately, there is a need to validate the concurrent imaging of the two elements to enhance the understanding of the dynamic progression of autophagy.

Methods

Probe HFI was synthesized in three steps and was developed to visualize changes in viscosity and pH within lysoso

SUBMITTER: Zheng F 

PROVIDER: S-EPMC10327318 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature