Transcriptional Profile of Human Pancreatic Acinar Ductal Metaplasia.

ABSTRACT:

Background and aims

Aberrant acinar to ductal metaplasia (ADM), one of the earliest events involved in exocrine pancreatic cancer development, is typically studied using pancreata from genetically engineered mouse models.

Methods

We used primary, human pancreatic acinar cells from organ donors to evaluate the transcriptional and pathway profiles during the course of ADM.

Results

Following 6 days of three-dimensional culture on Matrigel, acinar cells underwent morphological and molecular changes indicative of ADM. mRNA from 14 donors' paired cells (day 0, acinar phenotype and day 6, ductal phenotype) was subjected to whole transcriptome sequencing. Acinar cell specific genes were significantly downregulated in the samples from the day 6 cultures while ductal cell-speci

SUBMITTER: Jiang J 

PROVIDER: S-EPMC10328139 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature