Competition between myosin II and β_H-spectrin regulates cytoskeletal tension.

ABSTRACT: Spectrins are membrane cytoskeletal proteins generally thought to function as heterotetramers comprising two α-spectrins and two β-spectrins. They influence cell shape and Hippo signaling, but the mechanism by which they influence Hippo signaling has remained unclear. We have investigated the role and regulation of the Drosophila β-heavy spectrin (β_H-spectrin, encoded by the karst gene) in wing imaginal discs. Our results establish that β_H-spectrin regulates Hippo signaling through the Jub biomechanical pathway due to its influence on cytoskeletal tension. While we find that α-spectrin also regulates Hippo signaling through Jub, unexpectedly, we find that β_H-spectrin localizes and functions independently of α-spectrin. Instead, β_H-s

SUBMITTER: Ibar C 

PROVIDER: S-EPMC10328500 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature