High-throughput library transgenesis in <i>Caenorhabditis elegans</i> via Transgenic Arrays Resulting in Diversity of Integrated Sequences (TARDIS).

ABSTRACT: High-throughput transgenesis using synthetic DNA libraries is a powerful method for systematically exploring genetic function. Diverse synthesized libraries have been used for protein engineering, identification of protein-protein interactions, characterization of promoter libraries, developmental and evolutionary lineage tracking, and various other exploratory assays. However, the need for library transgenesis has effectively restricted these approaches to single-cell models. Here, we present Transgenic Arrays Resulting in Diversity of Integrated Sequences (TARDIS), a simple yet powerful approach to large-scale transgenesis that overcomes typical limitations encountered in multicellular systems. TARDIS splits the transgenesis process into a two-step process: creation of individuals carryi

SUBMITTER: Stevenson ZC 

PROVIDER: S-EPMC10328503 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature