The Opto-inflammasome in zebrafish as a tool to study cell and tissue responses to speck formation and cell death.

ABSTRACT: The inflammasome is a conserved structure for the intracellular detection of danger or pathogen signals. As a large intracellular multiprotein signaling platform, it activates downstream effectors that initiate a rapid necrotic programmed cell death (PCD) termed pyroptosis and activation and secretion of pro-inflammatory cytokines to warn and activate surrounding cells. However, inflammasome activation is difficult to control experimentally on a single-cell level using canonical triggers. We constructed Opto-ASC, a light-responsive form of the inflammasome adaptor protein ASC (Apoptosis-Associated Speck-Like Protein Containing a CARD) which allows tight control of inflammasome formation in vivo. We introduced a cassette of this construct under the control of a heat shock element into zebra

SUBMITTER: Hasel de Carvalho E 

PROVIDER: S-EPMC10328535 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature