SARS-CoV-2 spike protein reduces burst activities in neurons measured by micro-electrode arrays.

ABSTRACT: Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) caused a large-scale global pandemic between 2020 and 2022. Despite efforts to understand its biological and pathogenic mechanisms, the viral impact on the neurological systems remains unclear. The main goal of this study was to quantify the neurological phenotypes induced by the SARS-CoV-2 spike protein in neurons, as measured by in-vitro multiwell micro-electrode arrays (MEAs).

Materials and methods

The authors extracted the whole-brain neurons from the newborn P1 mice and plated them on multiwell MEAs and administered purified recombinant spike proteins (both S1 and S2 subunits) from the SARS-CoV-2 virus. The signals from the MEAs were transmitted from an amplifier to a high-performance computer for recording and

SUBMITTER: Salvador M 

PROVIDER: S-EPMC10328704 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature