NMR metabolite quantification of a synthetic urine sample: an inter-laboratory comparison of processing workflows.

ABSTRACT:

Introduction

Absolute quantification of individual metabolites in complex biological samples is crucial in targeted metabolomic profiling.

Objectives

An inter-laboratory test was performed to evaluate the impact of the NMR software, peak-area determination method (integration vs. deconvolution) and operator on quantification trueness and precision.

Methods

A synthetic urine containing 32 compounds was prepared. One site prepared the urine and calibration samples, and performed NMR acquisition. NMR spectra were acquired with two pulse sequences including water suppression used in routine analyses. The pre-processed spectra were sent to the other sites where each operator quantified the metabolites using internal referencing or external calibration, and his/her favourit

SUBMITTER: Canlet C 

PROVIDER: S-EPMC10328857 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature