A novel CTLA-4 blocking strategy based on nanobody enhances the activity of dendritic cell vaccine-stimulated antitumor cytotoxic T lymphocytes.

ABSTRACT: Despite the great success of CTLA-4 blocking in cancer treatment, the use of anti-CTLA-4 monoclonal antibodies still faces many limitations. Now, immune checkpoint blocking coupled with adoptive cell therapy is gaining much attention. In this paper, we reported a strategy on the basis of anti-CTLA-4 nanobody (Nb)-modified liposomes to improve these obstacles. An Nb36/liposome complex was constructed and utilized as a blocker of the CTLA-4/B7 signal pathway in a combination with dendritic cell (DC)/tumor fusion vaccine to enhance the CD8⁺ T cell cytokine secretion, activation, proliferation, as well as specific cytotoxicity. Moreover, the CD8⁺ T cells induced by LPS-Nb36 and DC/tumor fusion vaccine led to higher CD8⁺ T cell effector function in vivo, which s

SUBMITTER: Yang W 

PROVIDER: S-EPMC10328924 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature