Metagenomic surveillance for bacterial tick-borne pathogens using nanopore adaptive sampling.

ABSTRACT: Technological and computational advancements in the fields of genomics and bioinformatics are providing exciting new opportunities for pathogen discovery and genomic surveillance. In particular, single-molecule nucleotide sequence data originating from Oxford Nanopore Technologies (ONT) sequencing platforms can be bioinformatically leveraged, in real-time, for enhanced biosurveillance of a vast array of zoonoses. The recently released nanopore adaptive sampling (NAS) strategy facilitates immediate mapping of individual nucleotide molecules to a given reference as each molecule is being sequenced. User-defined thresholds then allow for the retention or rejection of specific molecules, informed by the real-time reference mapping results, as they are physically passing through a given sequenc

SUBMITTER: Kipp EJ 

PROVIDER: S-EPMC10328957 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature