Kinetochore component function in C. elegans oocytes revealed by 4D tracking of holocentric chromosomes.

ABSTRACT: During cell division, chromosome congression to the spindle center, their orientation along the spindle long axis and alignment at the metaphase plate depend on interactions between spindle microtubules and kinetochores, and are pre-requisite for chromosome bi-orientation and accurate segregation. How these successive phases are controlled during oocyte meiosis remains elusive. Here we provide 4D live imaging during the first meiotic division in C. elegans oocytes with wild-type or disrupted kinetochore protein function. We show that, unlike in monocentric organisms, holocentric chromosome bi-orientation is not strictly required for accurate chromosome segregation. Instead, we propose a model in which initial kinetochore-localized BHC module (comprised of BUB-1^Bub1, HCP-1/2

SUBMITTER: Pitayu-Nugroho L 

PROVIDER: S-EPMC10329006 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature