Membrane translocation process revealed by in situ structures of type II secretion system secretins.

ABSTRACT: The GspD secretin is the outer membrane channel of the bacterial type II secretion system (T2SS) which secrets diverse toxins that cause severe diseases such as diarrhea and cholera. GspD needs to translocate from the inner to the outer membrane to exert its function, and this process is an essential step for T2SS to assemble. Here, we investigate two types of secretins discovered so far in Escherichia coli, GspD_α, and GspD_β. By electron cryotomography subtomogram averaging, we determine in situ structures of key intermediate states of GspD_α and GspD_β in the translocation process, with resolution ranging from 9 Å to 19 Å. In our results, GspD_α and GspD_β present entirely different membrane interaction patterns and ways of tra

SUBMITTER: Yu Z 

PROVIDER: S-EPMC10329019 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature