Coherent diffractive imaging of proteins and viral capsids: simulating MS SPIDOC.

ABSTRACT: MS SPIDOC is a novel sample delivery system designed for single (isolated) particle imaging at X-ray Free-Electron Lasers that is adaptable towards most large-scale facility beamlines. Biological samples can range from small proteins to MDa particles. Following nano-electrospray ionization, ionic samples can be m/z-filtered and structurally separated before being oriented at the interaction zone. Here, we present the simulation package developed alongside this prototype. The first part describes how the front-to-end ion trajectory simulations have been conducted. Highlighted is a quadrant lens; a simple but efficient device that steers the ion beam within the vicinity of the strong DC orientation field in the interaction zone to ensure spatial overlap with the X-rays. The second part focus

SUBMITTER: Kierspel T 

PROVIDER: S-EPMC10329076 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature