Pressure-controlled microfluidics for automated single-molecule sample preparation.

ABSTRACT: Sample preparation is a crucial step in single-molecule experiments and involves passivating the microfluidic sample chamber, immobilizing the molecules, and setting experimental buffer conditions. The efficiency of the experiment depends on the quality and speed of sample preparation, which is often performed manually and relies on the experience of the experimenter. This can result in inefficient use of single-molecule samples and time, especially for high-throughput applications. To address this, a pressure-controlled microfluidic system is proposed to automate single-molecule sample preparation. The hardware is based on microfluidic components from ElveFlow and is designed to be cost-effective and adaptable to various microscopy applications. The system includes a reservoir pressure ad

SUBMITTER: Yang A 

PROVIDER: S-EPMC10329172 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature