Rapid and robust squashed spore/colony PCR of industrially important fungi.

ABSTRACT:

Background

Fungi have been utilized for centuries in medical, agricultural, and industrial applications. Development of systems biology techniques has enabled the design and metabolic engineering of these fungi to produce novel fuels, chemicals, and enzymes from renewable feedstocks. Many genetic tools have been developed for manipulating the genome and creating mutants rapidly. However, screening and confirmation of transformants remain an inefficient step within the design, build, test, and learn cycle in many industrial fungi because extracting fungal genomic DNA is laborious, time-consuming, and involves toxic chemicals.

Results

In this study we developed a rapid and robust technique called "Squash-PCR" to break open the spores and release fungal genomic DNA as a templat

SUBMITTER: Yuan G 

PROVIDER: S-EPMC10329332 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature