VA-TIRFM-based SM kymograph analysis for dwell time and colocalization of plasma membrane protein in plant cells.

ABSTRACT:

Background

The plasma membrane (PM) proteins function in a highly dynamic state, including protein trafficking and protein homeostasis, to regulate various biological processes. The dwell time and colocalization of PM proteins are considered to be two important dynamic features determining endocytosis and protein interactions, respectively. Dwell-time and colocalization detected using traditional fluorescence microscope techniques are often misestimated due to bulk measurement. In particular, analyzing these two features of PM proteins at the single-molecule level with spatiotemporal continuity in plant cells remains greatly challenging.

Results

We developed a single molecular (SM) kymograph method, which is based on variable angle-total internal reflection fluorescence micr

SUBMITTER: Su B 

PROVIDER: S-EPMC10329380 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature