Odontoblasts release exosomes to regulate the odontoblastic differentiation of dental pulp stem cells.

ABSTRACT:

Background

Dental pulp stem cells (DPSCs) play a crucial role in dentin-pulp complex regeneration. Further understanding of the mechanism by which DPSCs remain in a quiescent state could contribute to improvements in the dentin-pulp complex and dentinogenesis.

Methods

TSC1 conditional knockout (DMP1-Cre+; TSC1^f/f, hereafter CKO) mice were generated to increase the activity of mechanistic target of rapamycin complex 1 (mTORC1). H&E staining, immunofluorescence and micro-CT analysis were performed with these CKO mice and littermate controls. In vitro, exosomes were collected from the supernatants of MDPC23 cells with different levels of mTORC1 activity and then characterized by transmission electron microscopy and nanoparticle tracking analysis. DPSCs were coculture

SUBMITTER: Luo X 

PROVIDER: S-EPMC10329399 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature