USP51 promotes non-small cell lung carcinoma cell stemness by deubiquitinating TWIST1.

ABSTRACT:

Background

USP51 is a deubiquitinase (DUB), that is involved in diverse cellular processes. Accumulating evidence has demonstrated that USP51 contributes to cancer development. However, its impact on non-small cell lung carcinoma (NSCLC) cell malignancy is largely unknown.

Methods

In this study, we performed bioinformatics analysis on a dataset from The Cancer Genome Atlas to determine the association between USP51 and cell stemness marker expression in NSCLC patients. RT‒qPCR, Western blotting, and flow cytometry were performed to examine the effects of USP51 depletion on stemness marker expression. Colony formation and tumor sphere formation assays were used to assess the stemness of NSCLC cells. A cycloheximide chase time-course assay and a polyubiquitination assay were c

SUBMITTER: Chen J 

PROVIDER: S-EPMC10329790 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature