Discovery of a Phosphonoacetic Acid Derived Natural Product by Pathway Refactoring.

ABSTRACT: The activation of silent natural product gene clusters is a synthetic biology problem of great interest. As the rate at which gene clusters are identified outpaces the discovery rate of new molecules, this unknown chemical space is rapidly growing, as too are the rewards for developing technologies to exploit it. One class of natural products that has been underrepresented is phosphonic acids, which have important medical and agricultural uses. Hundreds of phosphonic acid biosynthetic gene clusters have been identified encoding for unknown molecules. Although methods exist to elicit secondary metabolite gene clusters in native hosts, they require the strain to be amenable to genetic manipulation. One method to circumvent this is pathway refactoring, which we implemented in an effort to dis

SUBMITTER: Freestone TS 

PROVIDER: S-EPMC10329809 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature