Identification of new resonances in downfield ¹ H MRS of human calf muscle in vivo: Potentially metabolite precursors for skeletal muscle NAD.

ABSTRACT:

Purpose

The purpose of this study was to identify and characterize newly discovered resonances appearing in the downfield proton MR spectrum (DF ¹ H MRS) of the human calf muscle in vivo at 7T.

Methods

Downfield ¹ H MRS was performed on the calf muscle of five healthy volunteers at 7T. A spectrally selective 90° E-BURP RF pulse with an excitation center frequency at 10.3 ppm and an excitation bandwidth of 2 ppm was used for DF ¹ H MRS acquisition.

Results

In all participants, we observed new resonances at 9.7, 10.1, 10.3, and 10.9 ppm in the DF ¹ H MRS. Phantom experiments at 37°C strongly suggest the new resonance at 9.7 ppm could be from H2-proton of the nicotinamide rings in nicotinamide riboside (NR) and nicotinamide mon

SUBMITTER: Nanga RPR 

PROVIDER: S-EPMC10330283 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature