Repeated mild traumatic brain injury in mice elicits long term innate immune cell alterations in blood, spleen, and brain.

ABSTRACT: Mild traumatic brain injury is an insidious event whereby the initial injury leads to ongoing secondary neuro- and systemic inflammation through various cellular pathways lasting days to months after injury. Here, we investigated the impact of repeated mild traumatic brain injury (rmTBI) and the resultant systemic immune response in male C57B6 mice using flow cytometric methodology on white blood cells (WBCs) derived from the blood and spleen. Isolated mRNA derived from spleens and brains of rmTBI mice was assayed for changes in gene expression at one day, one week, and one month following the injury paradigm. We observed increases in Ly6C+, Ly6C-, and total monocyte percentages in both blood and spleen at one month after rmTBI. Differential gene expression analysis for the brain and splee

SUBMITTER: Smith JA 

PROVIDER: S-EPMC10330602 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature