BONCAT-FACS-Seq reveals the active fraction of a biocrust community undergoing a wet-up event.

ABSTRACT: Determining which microorganisms are active within soil communities remains a major technical endeavor in microbial ecology research. One promising method to accomplish this is coupling bioorthogonal non-canonical amino acid tagging (BONCAT) with fluorescence activated cell sorting (FACS) which sorts cells based on whether or not they are producing new proteins. Combined with shotgun metagenomic sequencing (Seq), we apply this method to profile the diversity and potential functional capabilities of both active and inactive microorganisms in a biocrust community after being resuscitated by a simulated rain event. We find that BONCAT-FACS-Seq is capable of discerning the pools of active and inactive microorganisms, especially within hours of applying the BONCAT probe. The active and inactive

SUBMITTER: Trexler RV 

PROVIDER: S-EPMC10330726 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature