Assessing the influence of distinct culture media on human pre-implantation development using single-embryo transcriptomics.

ABSTRACT: The use of assisted reproductive technologies is consistently rising across the world. However, making an informed choice on which embryo culture medium should be preferred to ensure satisfactory pregnancy rates and the health of future children critically lacks scientific background. In particular, embryos within their first days of development are highly sensitive to their micro-environment, and it is unknown how their transcriptome adapts to different embryo culture compositions. Here, we determined the impact of culture media composition on gene expression in human pre-implantation embryos. By employing single-embryo RNA-sequencing after 2 or 5 days of the post-fertilization culture in different commercially available media (Ferticult, Global, and SSM), we revealed medium-specific diff

SUBMITTER: Ducreux B 

PROVIDER: S-EPMC10330962 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature