Extracellular matrix-derived peptide stimulates the generation of endocrine progenitors and islet organoids from iPSCs.

ABSTRACT: Induced pluripotent stem cells (iPSCs) have enormous potential in producing human tissues endlessly. We previously reported that type V collagen (COL5), a pancreatic extracellular matrix protein, promotes islet development and maturation from iPSCs. In this study, we identified a bioactive peptide domain of COL5, WWASKS, through bioinformatic analysis of decellularized pancreatic ECM (dpECM)-derived collagens. RNA-sequencing suggests that WWASKS induces the formation of pancreatic endocrine progenitors while suppressing the development of other types of organs. The expressions of hypoxic genes were significantly downregulated in the endocrine progenitors formed under peptide stimulation. Furthermore, we unveiled an enhancement of iPSC-derived islets' (i-islets) glucose sensitivity under pe

SUBMITTER: Heaton ES 

PROVIDER: S-EPMC10331343 | biostudies-literature | 2023 Jan-Dec

REPOSITORIES: biostudies-literature