Protocol for in vitro assessment of human monocyte transendothelial migration using a high-throughput live cell imaging system.

ABSTRACT: In vitro modeling of the different steps of immune cell recruitment is essential to decipher the role of endothelial cells in this process. Here, we present a protocol for the assessment of human monocyte transendothelial migration using a live cell imaging system. We describe steps for culture of fluorescent monocytic THP-1 cells and chemotaxis plate preparation with HUVEC monolayers. We then detail real-time analysis using the IncuCyte® S3 live-cell imaging system, image analysis, and assessment of transendothelial migration rates. For complete details on the use and execution of this protocol, please refer to Ladaigue et al.¹.

SUBMITTER: Ladaigue S 

PROVIDER: S-EPMC10331580 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature