Monospecies probiotic preparation and administration with downstream analysis of sex-specific effects on gut microbiome composition in mice.
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ABSTRACT:
SUBMITTER: Di Gesu CM
PROVIDER: S-EPMC10331592 | biostudies-literature | 2023 Jun
REPOSITORIES: biostudies-literature
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Monospecies probiotic preparation and administration with downstream analysis of sex-specific effects on gut microbiome composition in mice.
Di Gesù Claudia M CM Matz Lisa M LM Fultz Robert R Bolding Ian J IJ Buffington Shelly A SA
STAR protocols 20230627 3
Dysbiosis of the gut microbiome is implicated in the growing burden of non-communicable chronic diseases, including neurodevelopmental disorders, and both preclinical and clinical studies highlight the potential for precision probiotic therapies in their prevention and treatment. Here, we present an optimized protocol for the preparation and administration of Limosilactobacillus reuteri MM4-1A (ATCC-PTA-6475) to adolescent mice. We also describe steps for performing downstream analysis of metata ...[more]
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