Click chemistry-based thiol redox proteomics reveals significant cysteine reduction induced by chronic ethanol consumption.

ABSTRACT: In the U.S., alcohol-associated liver disease (ALD) impacts millions of people and is a major healthcare burden. While the pathology of ALD is unmistakable, the molecular mechanisms underlying ethanol hepatotoxicity are not fully understood. Hepatic ethanol metabolism is intimately linked with alterations in extracellular and intracellular metabolic processes, specifically oxidation/reduction reactions. The xenobiotic detoxification of ethanol leads to significant disruptions in glycolysis, β-oxidation, and the TCA cycle, as well as oxidative stress. Perturbation of these regulatory networks impacts the redox status of critical regulatory protein thiols throughout the cell. Integrating these key concepts, our goal was to apply a cutting-edge approach toward understanding mechanisms of etha

SUBMITTER: Harris PS 

PROVIDER: S-EPMC10331594 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature