Mef2a is a positive regulator of Col10a1 gene expression during chondrocyte maturation.

ABSTRACT:

Background

The type X collagen gene (Col10a1) is a signature gene of hypertrophic chondrocytes that are known as the main engine of long bone growth. Multiple transcription factors (TFs), including myocyte enhancer factor 2A (Mef2a), have previously been identified by in silico analysis as potential Col10al gene regulators.

Objectives

In this study, we aimed to investigate the correlation between Mef2a and Col10a1 expression and the possible effects on chondrocyte proliferation and hypertrophic differentiation in vitro.

Methods

First, Mef2a expression in proliferating and hypertrophic chondrocytes were detected by quantitative real-time PCR (qRT-PCR) and Western blotting in two chondrocytic models, ATDC5 and MCT cells, as well as in mouse c

SUBMITTER: Chen C 

PROVIDER: S-EPMC10331669 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature