CLEC5A regulates the proliferation and migration of colon cancer via the AKT/mTOR signaling pathway.

ABSTRACT:

Background

The purpose of this study is to understand the CLEC5A mechanism in colon cancer's proliferation and migration.

Methods

The CLEC5A expression levels in colon cancer tissues were analyzed using bioinformatics method based on Oncomine and The Cancer Genome Atlas (TCGA) databases, which were further tested by immunohistochemistry (IHC) and quantitative real-time polymerase chain reaction (qRT-PCR). The CLEC5A expression levels in 4 types of colon cancer cell lines (HCT116, SW620, HT29, and SW480) were also examined by qRT-PCR. We constructed CLEC5A knockdown cell lines and used colony formation, Cell Counting Kit-8 (CCK-8), 5-Ethynyl-2'-deoxyuridine (EdU), wound healing, and transwell assays for investigating the CLEC5A function in colon cancer's proliferation and mig

SUBMITTER: Pan Y 

PROVIDER: S-EPMC10331764 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature