Neutrophil-derived reactive agents induce a transient SpeB negative phenotype in Streptococcus pyogenes.

ABSTRACT:

Background

Streptococcus pyogenes (group A streptococci; GAS) is the main causative pathogen of monomicrobial necrotizing soft tissue infections (NSTIs). To resist immuno-clearance, GAS adapt their genetic information and/or phenotype to the surrounding environment. Hyper-virulent streptococcal pyrogenic exotoxin B (SpeB) negative variants caused by covRS mutations are enriched during infection. A key driving force for this process is the bacterial Sda1 DNase.

Methods

Bacterial infiltration, immune cell influx, tissue necrosis and inflammation in patient´s biopsies were determined using immunohistochemistry. SpeB secretion and activity by GAS post infections or challenges with reactive agents were determined via Western blot or casein agar and proteolytic activity assays, re

SUBMITTER: Shumba P 

PROVIDER: S-EPMC10331992 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature