γ-2 and GSG1L bind with comparable affinities to the tetrameric GluA1 core.

ABSTRACT:

Background

The AMPA-type ionotropic glutamate receptor mediates fast excitatory neurotransmission in the brain. A variety of auxiliary subunits regulate its gating properties, assembly, and trafficking, but it is unknown if the binding of these auxiliary subunits to the receptor core is dynamically regulated. Here we investigate the interplay of the two auxiliary subunits γ-2 and GSG1L when binding to the AMPA receptor composed of four GluA1 subunits.

Methods

We use a three-color single-molecule imaging approach in living cells, which allows the direct observation of the receptors and both auxiliary subunits. Colocalization of different colors can be interpreted as interaction of the respective receptor subunits.

Results

Depending on the relative expression levels of

SUBMITTER: Yu C 

PROVIDER: S-EPMC10332039 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature