Mettl3-mediated m⁶A modification plays a role in lipid metabolism disorders and progressive liver damage in mice by regulating lipid metabolism-related gene expression.

ABSTRACT:

Aims

N6-methyladenosine (m⁶A), the most abundant and conserved epigenetic modification of mRNA, participates in various physiological and pathological processes. However, the roles of m⁶A modification in liver lipid metabolism have yet to be understood entirely. We aimed to investigate the roles of the m⁶A "writer" protein methyltransferase-like 3 (Mettl3) in liver lipid metabolism and the underlying mechanisms.

Main methods

We assessed the expression of Mettl3 in liver tissues of diabetes (db/db) mice, obese (ob/ob) mice, high saturated fat-, cholesterol-, and fructose-induced non-alcoholic fatty liver disease (NAFLD) mice, and alcohol abuse and alcoholism (NIAAA) mice by quantitative reverse-transcriptase PCR (qRT-PCR). Hepatocyte-specific

SUBMITTER: Dai G 

PROVIDER: S-EPMC10333091 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature