Comparative proteomic profiling of uric acid, ammonium acid urate, and calcium-based kidney stones.

ABSTRACT:

Introduction

Kidney stone matrix proteins may help explain cellular mechanisms of stone genesis. However, most existing proteomic studies have focused on calcium oxalate stones. Here, we present a comparative proteomic analysis of different kidney stone types.

Methods

Proteins were extracted from the stones of patients undergoing percutaneous nephrolithotomy (PCNL). Approximately 20 μg of protein was digested into tryptic peptides using filter aided sample preparation, followed by liquid chromatography tandem-mass-spectrometry using an EASY-nLC 1200 and Orbitrap Fusion Lumos mass spectrometer. A standard false discovery rate cutoff of 1% was used for protein identification. Stone analysis was used to organize stone samples into similar groups. We selected the top 5% of prote

SUBMITTER: Khusid JA 

PROVIDER: S-EPMC10333134 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature