Derivation of embryonic stem cells from wild-derived mouse strains by nuclear transfer using peripheral blood cells.

ABSTRACT: Wild-derived mouse strains have been extensively used in biomedical research because of the high level of inter-strain polymorphisms and phenotypic variations. However, they often show poor reproductive performance and are difficult to maintain by conventional in vitro fertilization and embryo transfer. In this study, we examined the technical feasibility of derivation of nuclear transfer embryonic stem cells (ntESCs) from wild-derived mouse strains for their safe genetic preservation. We used leukocytes collected from peripheral blood as nuclear donors without sacrificing them. We successfully established 24 ntESC lines from two wild-derived strains of CAST/Ei and CASP/1Nga (11 and 13 lines, respectively), both belonging to Mus musculus castaneus, a subspecies of laboratory mouse. Most (2

SUBMITTER: Watanabe N 

PROVIDER: S-EPMC10333218 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature