Nanotubes from bacteriophage tail sheath proteins: internalisation by cancer cells and macrophages.

ABSTRACT: Bionanoparticles comprised of naturally occurring monomers are gaining interest in the development of novel drug transportation systems. Here we report on the stabilisation, cellular uptake, and macrophage clearance of nanotubes formed from the self-assembling gp053 tail sheath protein of the vB_EcoM_FV3 bacteriophage. To evaluate the potential of the bacteriophage protein-based nanotubes as therapeutic nanocarriers, we investigated their internalisation into colorectal cancer cell lines and professional macrophages that may hinder therapeutic applications by clearing nanotube carriers. We fused the bacteriophage protein with a SNAP-tag self-labelling enzyme and demonstrated that its activity is retained in assembled nanotubes, indicating that such carriers can be applied to deliver therap

SUBMITTER: Gabrielaitis D 

PROVIDER: S-EPMC10334369 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature