Construction and characterization of a novel miniaturized filamentous phagemid for targeted mammalian gene transfer.

ABSTRACT:

Background

As simplistic proteinaceous carriers of genetic material, phages offer great potential as targeted vectors for mammalian transgene delivery. The filamentous phage M13 is a single-stranded DNA phage with attractive characteristics for gene delivery, including a theoretically unlimited DNA carrying capacity, amenability to tropism modification via phage display, and a well-characterized genome that is easy to genetically modify. The bacterial backbone in gene transfer plasmids consists of elements only necessary for amplification in prokaryotes, and, as such, are superfluous in the mammalian cell. These problematic elements include antibiotic resistance genes, which can disseminate antibiotic resistance, and CpG motifs, which are inflammatory in animals and can lead to tra

SUBMITTER: Wong S 

PROVIDER: S-EPMC10334589 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature