Extracellular vesicles improve GABAergic transmission in Huntington's disease iPSC-derived neurons.

ABSTRACT: Background: Extracellular vesicles (EVs) carry bioactive molecules associated with various biological processes, including miRNAs. In both Huntington's disease (HD) models and human samples, altered expression of miRNAs involved in synapse regulation was reported. Recently, the use of EV cargo to reverse phenotypic alterations in disease models with synaptopathy as the end result of the pathophysiological cascade has become an interesting possibility. Methods: Here, we assessed the contribution of EVs to GABAergic synaptic alterations using a human HD model and studied the miRNA content of isolated EVs. Results: After differentiating human induced pluripotent stem cells into electrophysiologically active striatal-like GABAergic neurons, we found that HD-derived neurons

SUBMITTER: Beatriz M 

PROVIDER: S-EPMC10334823 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature