In vitro-transfusional model for red-blood-cell study: the advantage of lowering hematocrit.

ABSTRACT:

Background

The quality of red blood cells (RBCs) stored in red cell concentrates (RCCs) is influenced by processing, storage and donor characteristics, and can have a clinical impact on transfused patients. To evaluate RBC properties and their potential impact in a transfusion setting, a simple in vitro-transfusional model has been developed.

Materials and methods

Transfusion was simulated by mixing a washed RBC pool from two male-derived RCCs stored at 4°C with a pool of 15 male-derived fresh frozen plasma (FFP) units, representing the recipient, at a hematocrit (HCT) of 30% ("control" setting) or 5% (alternative model). The mixtures were incubated at 37°C, 5% of CO₂ up to 48 h. Different metabolites, hemolysis and microvesicles (MVs) were quantified at several i

SUBMITTER: Langst E 

PROVIDER: S-EPMC10335337 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature