The type-III effectors-based multiplex PCR for detection of <i>Xanthomonas campestris</i> pv. <i>campestris</i> causing black rot disease in crucifer crops.

ABSTRACT: The black rot disease in crucifer crops is caused by Xanthomonas campestris pv. campestris (Xcc) which drastically reduces the productivity of crops. Three Xcc races, such as races 1, 4, and 6, have been identified from India that possess nine avr genes, or type-III effectors (T3Es). Here, we used three T3Es-avrXccC, avrBs1, and avrGf1 to identify Xcc from bacterial DNA, bacterial suspensions, Xcc-infected seeds, and the sap of the infected leaves using multiplex PCR. The T3Es were amplified using gene-specific primers with gDNA of Xcc. Then, the multiplex PCR was optimized and amplified T3Es using the sap of black rot-infected cauliflower leaves. Further, this method amplified T3Es from artificially infected seeds (1-100%) of cauliflower and from Xcc colonies (

SUBMITTER: Singh D 

PROVIDER: S-EPMC10335992 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature