An RNA modification enzyme directly senses reactive oxygen species for translational regulation in Enterococcus faecalis.

ABSTRACT: Bacteria possess elaborate systems to manage reactive oxygen and nitrogen species (ROS) arising from exposure to the mammalian immune system and environmental stresses. Here we report the discovery of an ROS-sensing RNA-modifying enzyme that regulates translation of stress-response proteins in the gut commensal and opportunistic pathogen Enterococcus faecalis. We analyze the tRNA epitranscriptome of E. faecalis in response to reactive oxygen species (ROS) or sublethal doses of ROS-inducing antibiotics and identify large decreases in N²-methyladenosine (m²A) in both 23 S ribosomal RNA and transfer RNA. This we determine to be due to ROS-mediated inactivation of the Fe-S cluster-containing methyltransferase, RlmN. Genetic knockout of RlmN gives rise to a proteome that m

SUBMITTER: Lee WL 

PROVIDER: S-EPMC10336011 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature