Myeloid Phenotypes in Tracheostomy-Associated Granulation Tissue.

ABSTRACT:

Objective(s)

Tracheostomy-associated granulation tissue is a common, recurrent problem occurring secondary to chronic mucosal irritation. Although granulation tissue is composed of predominantly innate immune cells, the phenotype of monocytes and macrophages in tracheostomy-associated granulation tissue is unknown. This study aims to define the myeloid cell population in granulation tissue secondary to tracheostomy.

Methods

Granulation tissue biopsies were obtained from 8 patients with tracheostomy secondary to laryngotracheal stenosis. Cell type analysis was performed by flow cytometry and gene expression was measured by quantitative real-time polymerase chain reaction. These methods and immunohistochemistry were used to define the monocyte/macrophage population in granulat

SUBMITTER: Berges AJ 

PROVIDER: S-EPMC10336175 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature