Practical considerations for accurate determination of free thyroxine by equilibrium dialysis.

ABSTRACT:

Background

Free thyroxine (FT4) measurement is one of the most requested tests in patient care for diagnosing and treating thyroid-related illnesses. Equilibrium dialysis (ED) is considered the "gold standard" for FT4 measurement; however, several factors have a profound effect on the reliability of FT4 assays and require special consideration.

Methods

In the current study, we focused on evaluating critical factors that could contribute to reporting errors, such as adsorption of thyroxine (T4) to labware surfaces, stability of serum samples, stock solutions, and calibrator storage conditions, as well as the solvents used to prepare T4 solutions.

Results

The adsorption of T4 in ethanolic solutions and dialysates to labware surfaces can be reduced with the careful selec

SUBMITTER: Ribera A 

PROVIDER: S-EPMC10336244 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature